Inoculation, Purification and Serology of Tobacco Leaf Curl Virus
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چکیده
منابع مشابه
Natural recombination between Tomato yellow leaf curl virus-is and Tomato leaf curl virus.
The complete genome sequences (2791 and 2793 nt) of isolates of Tomato yellow leaf curl virus-Is (TYLCV-Is) from Spain (SP72/97) and Portugal (Port2/95) were determined. These isolates are closely related to TYLCV-Is isolates reported in Japan (Japan-A and Japan-S) and Israel (Israel/Mild). Comparison of all sequenced isolates of TYLCV-Is showed that part of the genome comprising the intergenic...
متن کاملBiolistic inoculation of plants with tomato yellow leaf curl virus DNA.
Tomato yellow leaf curl virus (TYLCV) full-length DNA was amplified by PCR and cloned into a bacterial plasmid. The cloned TYLCV DNA was excised from the plasmid, ligated and the resulting monomeric circular double-stranded TYLCV DNA was used to inoculate tomato (Solanum lycopersicom) and datura (Datura stramonium) plants by particle bombardment. The bombarded plants produced typical disease sy...
متن کاملPurification of Tomato leaf curl Bangalore virus and production of polyclonal antibodies
Tomato leaf curl Bangalore virus (ToLCBV) was successfully transmitted using viruliferous whiteflies (Bemisia tabaci) to several tomato varieties and hybrids, viz. Arka vikas, Pusa ruby, Rashmi, Rakshitha and Swaraksha. Hybrid Rashmi took 100% uniform infection, which was selected for further propagation of the virus. ToLCBV was also transmitted to Nicotiana benthamiana; the rate of infection w...
متن کاملidentification of tobacco leaf curl virus infecting lonicera japonica, an ornamental plant common in japan
a begomovirus, tentatively named tobacco leaf curl japan virus-jpu (tblcjv-jpu), was isolated from lonicera japonica (honeysuckle) plants grown in utsunomiya showing veinal chlorosis symptoms. the tblcjv-jpu genome with 2,761 nt showed a highest identity with tblcjv-jp3 and was also close to tblcjv and as well to tblcjv-jp2. the overall nt identity with tblcjv-jp3 amounted to 92.94%, while the ...
متن کاملPhenotypic and Molecular Screening of Tomato Germplasm for Resistance to Tomato Yellow Leaf Curl Virus
Tomato yellow leaf curl virus (TYLCV) is a major tomato virus in tropical and subtropical regions. In this study, 134 accessions of Solanum lycopersicum and six accessions of Solanum peruvianum were assessed for resistance to an Iranian isolate of TYLCV. Plants were inoculated using whiteflies (Bemisia tabaci) and the reaction of plants was evaluated based on either disease symptoms or viral DN...
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ژورنال
عنوان ژورنال: Japanese Journal of Phytopathology
سال: 1972
ISSN: 1882-0484,0031-9473
DOI: 10.3186/jjphytopath.38.323